一种启动子OsP006、制备方法及应用

Promoter OsP006 as well as preparation method and application thereof

Abstract

本发明涉及一种启动子OsP006、制备方法及应用。所述启动子含有选自以下任意一组并具有启动子功能的核苷酸序列:a、Seq ID No.1所示的核苷酸序列;b、与Seq ID No.1互补的核苷酸序列;c、在高等严紧条件下能够与上述a或b的核苷酸序列杂交的核苷酸序列;d、对上述a或b所示核苷酸序列进行一个或多个碱基的取代、缺失、添加修饰的核苷酸序列;e、与上述a或b所示核苷酸序列具有至少90%同一性的核苷酸序列。本发明启动子能够在单子叶和双子叶植物中调控基因表达,从而为研究植物中目的基因的表达提供了一种新的工具和选择。
The invention relates to a promoter OsP006 as well as a preparation method and an application thereof. The promoter OsP006 contains one of the following nucleotide sequences with the promoter functions: a. the nucleotide sequence shown by Seq ID No.1; b. the nucleotide sequence being complementary to the Seq ID No.1; c. the nucleotide sequence capable of interbreeding with the nucleotide sequence of a or b under an advanced strict condition; d. the nucleotide sequence obtained by replacing, deleting, adding and modifying one or more bases in the nucleotide sequence of a or b; and e. the nucleotide sequence having at least 90% identity with the nucleotide sequence of a or b. The promoter OsP006 can be used for regulating the gene expression in a monocotyledon and dicotyledon, thus a new tool and choice are provided for the research of the expression of target genes in plants.

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