Improved method for extracting insulin from animal pancreas



The invention provides an improved method for extracting insulin from animal pancreas. The technical scheme adopted is that the method comprises the following steps: adopting an acid alcohol fluid method to damage the pancreas for extracting the insulin; adjusting pH according to an isoelectric precipitation principle; performing alkalifying and acidation; removing impure protein; carrying decompression concentration for further purifying; removing ethanol; heating to remove ester; performing sodium chloride salting-out to prepare the insulin. Due to the adoption of the method, the insulin more than 49 g can be obtained from the pancreas of per kg.




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Cited By (1)

    Publication numberPublication dateAssigneeTitle
    CN-103739702-AApril 23, 2014青岛康原药业有限公司Method for extraction of insulin