Improved method for extracting insulin from animal pancreas

一种改良的从动物胰脏中提取胰岛素的方法

Abstract

本发明阐述一种改良的从动物胰脏中提取胰岛素的方法。本发明的技术方案采取酸醇液法破坏胰脏,来提取胰岛素,然后根据等电点沉淀的原理,调节pH,碱化、酸化除杂蛋白,减压浓缩进一步提纯并除去乙醇,最后加热去脂,氯化钠盐析制得胰岛素。本法每千克胰脏可得4g以上胰岛素。
The invention provides an improved method for extracting insulin from animal pancreas. The technical scheme adopted is that the method comprises the following steps: adopting an acid alcohol fluid method to damage the pancreas for extracting the insulin; adjusting pH according to an isoelectric precipitation principle; performing alkalifying and acidation; removing impure protein; carrying decompression concentration for further purifying; removing ethanol; heating to remove ester; performing sodium chloride salting-out to prepare the insulin. Due to the adoption of the method, the insulin more than 49 g can be obtained from the pancreas of per kg.

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Cited By (1)

    Publication numberPublication dateAssigneeTitle
    CN-103739702-AApril 23, 2014青岛康原药业有限公司Method for extraction of insulin