一种改性聚乙烯亚胺的制备方法

Preparation method of modified polyethyleneimine (PEI)

Abstract

The invention relates to a preparation method of modified polyethyleneimine (PEI), which comprises the following steps: (1) adding PEI into tri-distilled water, and heating and stirring in a water bath till dissolution to obtain a solution A, wherein the dosage ratio of PEI to tri-distilled water is 3g:(6-15mL); (2) introducing carbon dioxide (CO2) into the solution A, continuously bubbling for 3-8 hours at room temperature, and stirring to obtain a solution B; (3) freeze-drying the solution B and grinding to obtain modified PEI. The preparation method provided by the invention is quick, simple, efficient, cheap and convenient to operate, can be applied to gene transfection and biomedicine, and has a good application prospect.
本发明涉及一种改性聚乙烯亚胺的制备方法,包括:(1)将聚乙烯亚胺PEI加入三蒸水中,水浴加热搅拌至溶解,得到溶液A;其中聚乙烯亚胺PEI和三蒸水的用量比为3g:6-15mL;(2)将二氧化碳CO2通入溶液A中,室温条件下,持续鼓泡3-8h,搅拌,得到溶液B;(3)将上述溶液B冷冻干燥、研磨,即得。本发明快速、简捷、高效、廉价并且操作方便,可用于基因转染和生物医学方面,具有良好的应用前景。

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Patent Citations (3)

    Publication numberPublication dateAssigneeTitle
    CN-103007690-AApril 03, 2013浙江师范大学一种CO<sub>2</sub> 吸收剂及其制备方法
    CN-103122379-AMay 29, 2013深圳先进技术研究院基因转染实时监测的方法
    US-2012024153-A1February 02, 2012William Marsh Rice UniversityAliphatic amine based nanocarbons for the absorption of carbon dioxide

NO-Patent Citations (1)

    Title
    付新等: "聚乙烯亚胺修饰的介孔材料对C02的吸附性能", 《化工新型材料》, vol. 39, no. 11, 15 November 2011 (2011-11-15)

Cited By (2)

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    CN-104163920-ANovember 26, 2014东华大学Preparation method of transfection reagent for easy DNA combination
    CN-106192071-ADecember 07, 2016江苏鸿顺合纤科技有限公司一种具有粘接功能的复合纤维的制备方法